Mapping the Mouse Whole-Brain with Electron Microscopy
The Road to the Whole Brain
The path to mapping the complete circuit of the whole mouse brain is shown below.
We have developed methods for preparing the whole mouse brain for electron microscopy.
We are currently working on whole brain EM volume acquisition using Serial Blockface Electron Microscopy (SBEM) with a specially-designed whole-brain microtome (WBM).
Automated Circuit Reconstruction
With any luck, the whole mouse brain will be mapped ultrastructurally in the near term, which will then open the door to more serious problems; reliable automated segmentation and circuit reconstruction. These will undoubtedly require advances in machine learning methods and their application.
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